From Video games
Dante and Vergil (Devil May Cry)
Raiden (Metal Gear Rising)
Bayonetta (Bayonetta)
Gabriel Belmont/Dracula and Trevor Belmont/Alucard (Lords of Shadow)
John Marston (Red Dead Redemption)
Bigby Wolf (The Wolf Among Us)
Lee Everett (The Walking Dead S1)
Joel (The Last of Us)
From TV
Sam and Dean Winchester, Bobby Singer, and Castiel (Supernatural)
Buffy Summers, Angel, Spike, and Faith (Buffy the Vampire Slayer/Angel)
Klaus and Elijah Mikaelson (The Originals)
Jon Snow, Robb Stark, Tyrion Lannister (Game of Thrones)
Eric Northman (True Blood)
Derek Hale (Teen Wolf)
Ricky, Julian, and Bubbles (Trailer Park Boys......It's a Canadian show)
From Movies (This will also include comic book characters, since I don't read many comics)
Darth Vader (Star Wars)
Wolverine, Professor X, and Magneto (X-Men)
Batman, Joker, Bane, Commissioner Gordon, Alfred Pennyworth, and Lucius Fox (The Dark Knight Trilogy)
Michael Myers (Halloween)
Jason Voorhees (Friday the 13th)
Freddy Kruger (A Nightmare on Elm Street)
Leatherface (The Texas Chainsaw Massacre)
Blade (Blade Trilogy)
Iron Man, Captain America, Thor, and Hulk (The Avengers)
Star Lord, Rocket Raccoon, Gamora, Drax, and Groot (Guardians of the Galaxy)
The Terminator (The Terminator)
RoboCop (RoboCop)
Godzilla.....and various other giant monsters (Godzilla)
From Anime
Alucard, Alexander Anderson, and Seras Victoria (Hellsing, to be fair I love this whole cast, but this list is long enough)
Sesshomaru (Inuyasha)